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Art ID :258358(Ay)COMPONENTS STUDY ON GASTROPROTECTIVE EFFECT AND HOLISTIC MECHANISM OF THE HERBAL PAIR ALPINIA OFFICINARUM - CYPERUS ROTUNDUS BASED ON SPECTRUM-EFFECT RELATIONSHIP AND INTEGRATED TRANSCRIPTOME AND METABOLOME ANALYSES.

Journal of Ethnopharmacology
2024
321
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1,3. Huijuan Qu, 1. Ying Zhang, 1. Xiaomei Zhou, 1. Hongya Ou, 1,2. Kaiwen Lin, 1. Dejun Jin, 1. Yidan Kong, 2. Ning Ma, 1. Na Wei.
1. Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Key Laboratory for Research and Development of Tropical TCMs, School of Pharmacy, Hainan Medical University, Xueyuan Road 3, Haikou, Hainan Province, China. 2. Reproductive Medical Center, Hainan Women and Children's Medical Center, Longkun South Road 75, Haikou, Hainan Province, China. 3. Hainan Branch of Qilu Pharmaceutical Research Institute, Qilu Pharmaceutical Co., Ltd., Nanhai Avenue 273-A, Haikou, Hainan Province, China.
Ning Ma, Reproductive Medical Center, Hainan Women and Children's Medical Center, Longkun South Road 75, Haikou, Hainan Province, China. Email: maninggirl@163.com, Na Wei, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Key Laboratory for Research and Development of Tropical TCMs, School of Pharmacy, Hainan Medical University, Xueyuan Road 3, Haikou, Hainan Province, China. Email: weina-0613@163.com
ARP_AYU030861
JOURNAL ARTICLE
PRECLINICAL RESEARCH
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Abstract/ Summary/ Synopsis/ Outline:

Ethnopharmacological relevance: The herbal pair Alpinia officinarum-Cyperus rotundus (HPAC) has an extended history of use in the treatment of gastric ulcers, and its curative effect is definite. Aim of the study: To explore the material basis and holistic mechanism of HPAC on ethanol-induced gastric ulcers. Materials and methods: Three chemometrics, GRA, OPLS, and BCA, were used to construct the spectrum-effect relationship between the HPLC fingerprints of HPAC extracts and the bioactivity indices (cell viability; the levels of TNF-a, IL-6, COX-2, and PGE2; and wound healing rate) against GES-1 cell damage to screen the bioactive ingredients. The bioactive components were isolated and validated in vitro. Simultaneously, the effects of HPAC with concentrated bioactive ingredients was tested on ethanol-induced gastric ulcers in vivo, and the mechanism was investigated using transcriptomics and metabolomics. The mechanism was further validated by Western blotting. Finally, the contents of the main components of HPAC were determined before and after compatibility. Results: Twelve bioactive components were screened, and the structures of nine compounds were confirmed. An in vitro verification test showed that DPHA and galangin could protect GES-1 cells from injury, and that their content increased after compatibility. The CH2Cl2 fraction of HPAC (HP-CH2Cl2) can protect mice from ethanol-induced gastric mucosal injury by reducing hemorrhage and decreasing inflammatory cell infiltration. Western blot analysis indicated that this fraction may up-regulate TRPV1 protein and down-regulate PI3K and AKT proteins. Conclusions: DPHA and galangin may be the bioactive components against ethanol-induced GES-1 cell injury. HP-CH2Cl2 may exert gastroprotective effects by regulating PI3K, AKT and TRPV1 proteins.
Alpinia officinarum , Cyperus rotundus , Galangin , ethanol induced gastric ulcer
https://www.sciencedirect.com/science/article/abs/pii/S0378874123013648
10.1016/j.jep.2023.117494
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Acknowledgment

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